A spatial relationship between sheath elongation and cell proliferation in Sphaerotilus natans.
نویسندگان
چکیده
Sphaerotilus natans is a filamentous sheath-forming bacterium. A method of selective fluorescent-labeling of its sheath using conventional reagents was developed. Terminal expansion of the sheath was confirmed by this method. In addition, ubiquitous cell growth was revealed by sequential phase-contrast microscopy of a filament. Based on this and earlier reports, a model of the sheath formation is proposed.
منابع مشابه
Sphaerotilus natans encrusted with nanoball-shaped Fe(III) oxide minerals formed by nitrate-reducing mixotrophic Fe(II) oxidation
Ferrous iron has been known to function as an electron source for iron-oxidizing microorganisms in both anoxic and oxic environments. A diversity of bacteria has been known to oxidize both soluble and solid-phase Fe(II) forms coupled to the reduction of nitrate. Here, we show for the first time Fe(II) oxidation by Sphaerotilus natans strain DSM 6575(T) under mixotrophic condition. Sphaerotilus ...
متن کاملComposition of the Sheath of Sphaerotilus Natans.
Romano, Antonio H. (University of Cincinnati, Cincinnati, Ohio) and Joyce P. Peloquin. Composition of the sheath of Sphaerotilus natans. J. Bacteriol. 86:252-258. 1963.-The sheath of Sphaerotilus natans was isolated and subjected to chemical analysis. Isolation of the sheaths was accomplished by incubating cells in the presence of lysozyme and ethylenediaminetetraacetic acid in tris(hydroxymeth...
متن کاملPurification and properties of an enzyme capable of degrading the sheath of Sphaerotilus natans.
Microorganisms which can degrade and grow on the purified sheath of a sheathed bacterium Sphaerotilus natans were collected from soil and river water. Two bacterial strains were isolated from the soil and designated strains TB and TK. Both strains are rod shaped, negatively stained by gram staining, facultatively anaerobic, and formed ellipsoidal endospores. These characteristics suggested that...
متن کاملComposition of an extracellular poly-saccharide produced by Sphaerotilus natans.
The capsular polysaccharide of Sphaerotilus natans has been isolated, purified, and analyzed. Chromatographic and chemical analyses performed on acid hydrolyzates of the purified material have shown that the major components are fucose, galactose, glucose, and glucuronic acid in approximately equimolar amounts. Glucose and glucuronic acid are believed to occur as an aldobiuronic acid unit.
متن کاملIsolation and identification of the sudanophilic granules of Sphaerotilus natans.
Rouf, M. A. (Washington State University, Pullman) and J. L. Stokes. Isolation and identification of the sudanophilic granules of Sphaerotilus natans. J. Bacteriol. 83:343-347. 1962.-The characteristic and prominent sudanophilic granules in five strains of Sphaerotilus natans were shown to be composed of poly-beta-hydroxybutyric acid by the method of isolation, melting point determinations, and...
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عنوان ژورنال:
- Bioscience, biotechnology, and biochemistry
دوره 76 12 شماره
صفحات -
تاریخ انتشار 2012